Fig. 4

ERp29 deficiency potentiates platelet accumulation and fibrin deposition and venous thrombosis. (A) Representative images of platelet accumulation (Green) and fibrin generation (Red), as visualized by Dylight-488-conjugated anti-CD42c and Alexa-647-conjugated fibrin (59D8) antibody, respectively, at indicated time points following laser injury in the cremaster arterioles in ERp29^fl/fl and Pf4-Cre/ERp29^fl/fl mice. Scale bar, 30 μm. The median fluorescence intensity of platelets (B) and fibrin (C) were calculated and plotted over time. The area under the curve (AUC) for platelets (D) and fibrin (E) were analyzed from each individual thrombus in the two groups. Data are presented as Mean ± SEM and analyzed by two-tailed Mann–Whitney U-test, *P <.05. The data were obtained from 26 thrombi in 3 mice for each experimental condition. (F-H) Pf4-Cre/ERp29^fl/fl mice (n = 15) and control littermate ERp29^fl/fl mice (n = 14) were subjected to IVC stenosis for 48 h. Thrombi were extracted 48 h after surgery. (F) Representative image showing the different size of thrombi from ERp29^fl/fl and Pf4-Cre/ERp29^fl/fl mice after 48 h of stenosis. (G) Thrombus weight and (H) length at 48 h were quantified respectively. Mean ± SEM, **P <.01; ***P <.001, Student’s t test