Fig. 5
From: Endoplasmic reticulum protein 29 negatively regulates platelet functions and thrombosis in mice
ERp29 deficiency increases thiols of the αIIbβ3 integrin in activated platelets. (A-D) Thiols are increased in αIIbβ3 on the surface of activated ERp29 deficient platelets. Platelets from wild-type and ERp29 knockout mice were activated with thrombin (0.016 U/mL) for 5 min followed by MPB labeling. After platelet lysis, αIIbβ3 was immunoprecipitated. The labeled thiols were detected by blotting with fluorescence conjugated streptavidin. The membranes were re-probed with anti-αIIb and anti-β3 antibodies, and the MPB label was normalized to the protein density. Representative blot (A, C), cumulative data (B, D); Mean ± SEM, n = 3, *P <.05, Student’s t test. ns, not significant